Androgenesis—Technology for Obtaining Genetically Stable Breeding Material of Capsicum annuum L.

Androgenesis in vitro is a basic method of obtaining haploid plants and DH (doubled haploid) lines major crops such as potato, rapeseed, tomato, pepper, wheat, maize, barley, also many different minor species with lower agricultural impact. Diploid appearing among androgenic regenerants are the effect spontaneous doubling chromosome number cells during an embryo’s early developmental stages valuable fully homozygous breeding material. The subject presented research diploidization occurring development androgenic, pepper regenerants. In experiment, formation diploid seeds was observed progeny plant derived anther culture hybrid (Capsicum annuum L. ATZ × Capsicum `Corno di toro`)F2. Agromorphological molecular analyses concerned eight being anther-derived regenerant. Five constituted phenotypically balanced group agromorphological features. Their genetic homogeneity confirmed using 10 RAPD markers 16 ISSR markers. Based on results, it concluded that can be source diploid, apomictic seeds, obtained offspring may constitute genetically stable,